How are you doing the recording (i e are the recorder trigs OneShots)?
Is the sequencer running while you´re trimming and slicing this sample?
Or do you have the recorder trig in the beginning of the step sequencer?
That could be overwriting the buffer again: if you haven´t saved the sample inbetween, deactivated/dis-armed the recorder trig and loaded that sample (playback) at same step instead?